Comprehensive clinical studies carried out in Japan and France confirm the effect of taking Innovabio Collagen Drinks and highlight exciting new benefits on skin health.
A growing body of research is showing how nutricosmetics can contribute to healthy skin. While topical creams and cosmetic products can affect skin condition from the outside, nutritional supplements taken orally can have an impact from within the skin: this is called “Beauty from Within”.
To assess the efficacy and acceptability of Collagen Peptides, two double-blind randomized clinical studies versus placebo were carried on 80 healthy female subjects aged 35 to 59. The objective of these clinical studies was to evaluate the effect of oral intake of 10g of Collagen on dermophysiological measures and its tolerance.
Methodology
The latest investigative studies using state-of-the-art technology have challenged the effect of Innovabio Collagen Drinks. Two clinical studies were carried out by global independent skin health research organizations having a well proven approach for verifying product performance claims. Mechanical measurements and volunteers were assessed by certified skin experts.
These studies were performed according to local regulations on clinical studies and approved by the local Ethical Committee. Consent was obtained from each subject before entry in the study. Prior to each measurement, the volunteers were placed, for at least 20 minutes, in a room with controlled temperature and humidity.
Study YNTKK – 2008 – 4144
Double-blind placebo-controlled trial of oral supplementation of Collagen in improving skin condition. The study was conducted in Tokyo (Japan) by SOUKEN on 33 women from 40 to 59 years old for 8 weeks during winter. 10g of collagen were ingested once daily in liquid form.
Study 2008 – A00654 – 51
Evaluation of the effects of an oral intake of Collagen on cutaneous properties versus placebo. The study was conducted in Lyon (France) by DERMSCAN on 47 women from 35 to 55 years old, for 12 weeks during winter. 10g of Collagen were ingested, 5g in the morning and 5g at night, powder to mix with liquid.
Moisturizing effect measuring principle
Cutaneous hydration measurements were performed with a COURAGE & KHAZAKA Corneometer® CM 825. This instrument determines the humidity level of the most external cutaneous layers of the stratum corneum. The principle of the Corneometer® is based on the modification of the electrical capacity of the detector. The surface of the probe head, in contact with the skin, modifies its electrical capacity according to the humidity level of the skin.
Anti-aging effect measuring principles
Polymer silicone skin prints are taken on the studied area, before and at each time of measurement, then studied using the Skin Image Analyser® (SIA) or 3D Roughness analyzer ASA-03R (Asahi biomed co).
Oblique lighting (35°) brings out the relief of the replica that is then observed with a digital camera linked to a computer. A 1cm2 area is studied. This produces a digitized image enabling different parameters to be obtained by analyzing shades of grey.
Cutometer® by COURAGE & KHAZAKA was used with a 2mm probe to measure the way skin reacts to the mechanical constraints. This method measures the degree of deformation and the time required for the skin to return to its original state.
Results
Moisturizing effect (Study YNTKK – 2008 – 4144)
Collagen increases skin hydration.
There was a significant improvement for the Collagen test group of skin hydration by 28% compared to placebo after 8 weeks.
The skin hydration level of 91% of Collagen volunteers increased after 8 weeks.
Anti-aging effect (Study 2008 – A00654 – 51)
Collagen smoothes skin micro-relief furrows and prevents deep wrinkle formation.
The number of micro-relief furrows significantly decreases with Collagen: -26% after 12 weeks.
The number of deep wrinkles significantly increases with the placebo between baseline and week 12: +30%.
Collagen increases skin suppleness.
The skin suppleness increases by 19% in Collagen group.
After 6 weeks,77% of Collagen group volunteers had their micro-relief furrows decreased by 41% and at 12 weeks, we measured a decrease of the total wrinkled surface of 17% for 68% of the group.
Conclusion
These two clinical studies demonstrated the benefits of Collagen on skin health.
Taken daily, up to 12 weeks, the results verify that regular intake of Collagen improves the basic skin condition and structure.
Thanks to these results and its regulatory status, Collagen represents a very potent ingredient for the nutricosmetic market.
Collagen has clinically shown to:
- Improve skin moisture level
- Improve skin smoothness by reducing the number of micro-relief furrows
- Prevent the formation of deep-wrinkles
- Improve skin suppleness
Collagen is a completely characterized and scientifically objectivized natural bioactive ingredient that improves epidermis moisture content and prevents skin aging.
Mechanism of action
This commentary compiles the available scientific information, existing literature and Collagen clinical studies results. Collagen is a completely characterized and objectivized bioactive ingredient that improves epidermis moisture content and prevents skin aging.
Collagen peptides from fish, porcine or bovine origin is currently used in various fields including functional food, beverages and dietary supplements. Several studies have demonstrated that Collagen peptides is highly digestible. If native collagen is very resistant and regarded as indigestible, Collagen peptides can be easily attacked by proteolytic enzymes. More than 90% of the hydrolysates are digested and quickly absorbed after oral ingestion (2,3). As a food ingredient, oral ingestion of Collagen peptides has been reported as safe (1).
In order to be active, Collagen peptides must have an excellent bioavailability. This has been confirmed in animals and human after oral administration: 95% was absorbed within the first 12h. Radioactivity in cartilage attained its peak value 12h after the oral administration of 14C labeled Collagen peptides, and in contrasts to plasma, 14C-activity remained relatively high after 96h (3). Collagen peptides derived hydroxyproline peptides appeared in human blood after 12h fasting. Their amount increased after Hydrolyzed Collagen intake with a peak level after 2h and then decreased to half after 4h from the ingestion. Proline-Hydroxyproline (Pro-Hyp) can be considered as one of the indigestible peptide against peptidase in human blood since 75% of Pro-Hyp remained after the in vitro reaction with human serum for 24h (4).
When type I collagens are digested by collagenase, the resulting peptides are chemo-attractants for fibroblasts. Chemotactic response of dermal fibroblasts of collagen derived peptide was quantified through an in vitro assay.
In addition, synthetic di- and tri- Hyp containing peptides were also chemotactic. Collagen peptides may act as messenger and trigger the synthesis and reorganization of new collagen fibers by stimulating fibroblast cells (5).
Furthermore, some studies show that Collagen peptides increases the fibroblast density and the diameter of collagen fibrils in the dermis.
Collagen peptides may improve the mechanical strength of the skin by increasing decorin ratio (6,7). Decorin interacts with collagen and influences collagen fibrillogenesis, thus regulating excessive bundle-like aggregation of collagen.
Clinical studies show that increases skin suppleness which could come from the better cohesion of collagen fibers. Some clinical studies have shown that the oral intake of 5 to 10g per day of Collagen peptides may have a positive effect on human tissues containing collagen such as skin. The moisture content of forearms and backs of the necks increased significantly. This implies that ingestion of Collagen peptides improves the function of the outermost part of the epidermis. Skin‘s relief was also improved (8,9,10). Those results were perfectly in line with Innovabio Collagen clinical studies: improves skin’s hydration and smoothness. Therefore it might boost epidermis cells turnover, speeding the water moves through the skin layer, improving the water-binding function of the outermost part of the epidermis and preventing the formation deep-wrinkles by stimulating collagen synthesis.
References
1. Wu, J., Fujioka, M., Sugimoto, K., Mu, G. and Ishimi, Y. 2004. Assessment of effectiveness of oral administration of collagen peptide on bone metabolism in fgrowing and mature rats. Journal of bone and mineral metabolism, 22:47-553.
2. Asghar, A and Henrickson, R.L. 1982. Chemical, biochemical, functional characteristics of collagen in food system. Advances in food research, 28 :231-372.
3. Oesser, S., Adam, M., Babel, W. and Seifert, J. 1999. Oral administration of 14C labeled gelatinhydrolysate leads to an accumulation of radioactivity in cartilage of mice (C57/BL). Journal of nutrition, 129:1891-1895.
4. Iwai, K., Hasegawa, T., Taguchi, Y., Morimatsu, F., Sato, K., Nakamura, Y., Higashi, A., Kido, Y., Nakabo, Y. and Ohtsuki, K. 2005. Identification of food-derived collagen peptides in human blood after oral ingestion of gelatin hydrolysates. Journal of agricultural and food chemistry, 53: 6531-6536.
5. Postlethwaite, A.E., Seyer, J.M., and Kang, A.H. 1978. Chemotactic attraction of human fibroblasts to type I, II, and II collagens and collagen-derived peptides. Proceedings of the National Academy of Sciences of the United States of America. 75(2): 871-875.
6. Minaguchi, J., Koyama, Y-I., Meguri, N., Hosaka, Y., Ueda, H., Kusubata, M., Hirota, A., Irie, S., Mafune, N. and Takehana, K. 2005. Effects of ingestion of collagen peptide on collagen fibrils and glycosaminoglycans in Achilles tendon. Journal of nutritional science and vitaminology 51:169-174.
7. Matsuda, N., Koyama, Y-I., Hosaka, Y., Ueda, H., Watanabe, T., Araya, S., Irie, S. and Takehana, K. 2006. Effects of ingestion of collagen peptide on collagen fibrils and glycosaminoglycans in the dermis. Journal of nutritional science and vitaminology. 52: 211-215.
8. Matsumoto, H., Ohara, H., Ito, K., Nakamura, Y. and Takahashi, S. 2006. Clinical effects of fish type I collagen hydrolysate on skin properties. ITE Letters on batteries, new technologies and medicine, 7(4):386-390.
9. Sumida, E., Hirota, A., Kuwaba, K., Kusubata, M., Koyama, Y., Araya, T. Irie, S. and Kasugai, S. 2004. The effect of oral ingestion of collagen peptide on skin hydration and biochemical data of blood. Journal of nutritional food, 7(3): 45-52.
10. Morganti, P., Randazzo, S.D. and Bruno, C. 1988. Oral treatment of skin dryness. Cosmetics and Toiletries, 103: 77-80.